FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872095
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56410.3013201787302461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG3650.021.7387317
GGCTCGG3600.020.124085
GCTCGGA4000.018.9741346
TATACAG10450.017.4968095
CTAATAC7950.017.3572353
TCTATAC7700.017.0247423
GTATTAG5650.015.8758061
ACCGCGC2001.1429282E-515.5247058
TATTAGG5600.015.4006712
TGGACCG3151.3587851E-915.3326325
GGACCGT2252.050354E-615.3326326
TATTATA9950.015.2551772
TATATAC13700.015.1083953
TAATACT11350.014.8931944
ACAGTGC5400.014.6941668
GTACTAA5900.014.6183661
GGGCTCG4251.8189894E-1214.6107084
GTATAAT9000.014.18306351
CTATACA8350.014.0468874
TATAATA11700.013.8579712
Produced by FastQC (version 0.11.2)