FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72046
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11211.555950365044555No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC150.002348254268.98758
ACGCGAA200.007316309551.77658518
TGGCGCC200.007316309551.77658517
TGCATGG200.00733645351.74062732
AACGCGG200.00733645351.74062764
GGTGCCT200.00733645351.74062710
GACAGTA306.2775257E-445.991677
TATACAG350.00134062139.421435
TACGCGT557.3075025E-637.62954769
GTATATA605.37822E-428.7447931
ACAATAC500.007694449727.5950013
TTACGCG756.0923958E-527.59568
CTTACGC1004.2859293E-420.6962567
TCTTACG1250.001919236516.55766
GCCGACG3454.623871E-913.99746369
TGCCGAC3902.5778718E-812.38237368
ACGATCT2357.583735E-411.74255369
GACGATC2409.048518E-411.49791768
CTGCCGA4258.519419E-811.36264767
GCTGCCG4803.90919E-810.77929766
Produced by FastQC (version 0.11.2)