FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653321
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151900.4157860751902174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA15700.021.7534221
GTATTAG13650.020.9767151
GCGACTC7200.016.28931434
TAGGTAG7450.015.7448465
CCTAGCG7550.015.53375530
CTAGGCA7800.015.48022710
TATACAG19500.014.8614265
CGATCAC8200.014.72408924
TATTAGG14100.014.4350742
GACTAGG8250.014.218278
CCGCTCG1951.5997226E-414.1539349
AGACTAG8700.013.87939557
CTAATAC13800.013.7495193
TCTATAC15750.013.5804793
TATTATA22100.013.2682232
TTAGGAC10250.013.1263713
GTCCTAC4751.6370905E-1113.072871
CACCTAG9500.013.071259528
GTATTAT23500.013.0651431
TATAGAG17700.013.058332
Produced by FastQC (version 0.11.2)