FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826952
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35140.4249339744023837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG500.007732296427.60101354
GTATAAT2155.456968E-1222.4645861
GTATTAA1854.838512E-1022.377851
CGGGTAT1004.328778E-420.70326460
CGTCTAG850.004119784620.2936381
TTAGTAC2603.6379788E-1219.9033763
CCGTGCA1056.0219906E-419.713829
TATACAG2801.2732926E-1118.4817075
TATAGAA2701.5097612E-1017.8884662
TATAAGC1200.001476537317.2495924
GTTATAG1804.194253E-617.2495921
TCCTATA1250.001940298716.5596082
GTATATA2109.96888E-716.4281831
GTATAGA2804.7239155E-916.0174791
GTAATAA2603.2969183E-815.92271
CCTATTA1300.00252130715.92272
AACGGGG1300.002522324415.92173734
CCTTATA1756.354441E-515.7710542
GTGTGCG1554.523021E-415.5802769
ATACAGA3101.0804797E-915.5802766
Produced by FastQC (version 0.11.2)