FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895344
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36680.19352687427717608No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTG900.005735331719.1669149
GTATTAG6250.018.768741
CGCGTGA950.00783688518.15765210
TATACAG7650.017.5884655
CCTAATA4500.016.0997852
TATTATA7300.015.5956812
GTATTAA6250.015.4566091
TATTAGG5600.015.401582
GTATTAT7250.015.2281861
GTATAAT8000.015.0943451
TATACCG4400.014.8979225
GTATATA8250.014.6369391
CTAATAC7100.014.5776543
TTATATA6400.014.5544922
TAATACT9500.014.5265054
GTATAAA8550.014.12336351
GTCCTAT3454.9003575E-914.0005511
TATATAC10700.013.8646663
TAATACC8300.013.7170454
GTATAGA7900.013.5385071
Produced by FastQC (version 0.11.2)