FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953098
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82200.2079381791192629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13950.017.559381
TAGGTAG8400.016.4281715
CTAATAC15400.016.3534973
TATACAG19450.015.963625
CCTAATA14900.015.2813142
GTATAGA18350.015.0410761
TAATACT21850.014.9996344
CGCACCT8050.013.7153466
GGACCGC4304.1836756E-1113.6392036
ACTGTGC11500.013.4996718
TTATACT17700.013.4488254
TATTAGG14000.013.306652
GTATATA26150.013.1932961
TATACCG10000.013.1096815
TATTATA22300.012.9950332
GACCGCG4254.984031E-1012.9879187
GTATAAG15450.012.9516321
TACACCG8350.012.8080715
GTATATG17500.012.6173141
GACGCGC5205.456968E-1212.60418638
Produced by FastQC (version 0.11.2)