FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165790
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84030.20171444071832711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC31450.014.4793993
GTATATA30050.014.2362411
GTATAGA20150.014.2108861
TACACCG10300.013.7326495
TTATACT24750.013.5206944
TATACTG22550.013.463065
CCTATAC11150.012.99486353
TAATACT23550.012.7447364
TCTATAC19650.012.6405863
TATTATA21200.012.3673032
GTATTAT21250.012.01409151
GTATTAG17050.011.938391
CTAGTAC16800.011.9101153
GTATAAA23750.011.7662891
ATAATAC22950.011.4242623
GTCTAGG7600.011.3486591
ATATACT31950.011.3375944
CTATATA21000.011.3351442
CGCGTGC2752.2722752E-411.28925733
GTATAAT21950.011.1594281
Produced by FastQC (version 0.11.2)