FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273305
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4640.16977369605385925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCGT400.00259454134.49551810
TTATACT1251.2369128E-1030.3560564
TAACCAT851.4406441E-424.3497755
TATACTT1701.6552804E-1024.3497755
TATACTG1001.5595726E-524.1468625
GATATAC750.00199066422.9970113
TATAAGG750.00199066422.9970113
AGGGAGA1103.2584452E-521.9516936
ATATTAA953.0600428E-421.7866421
CTTTAGA800.002896025321.5596982
CTAAGAT850.00411537520.2914813
TATCCTT1701.0788244E-720.2914815
TTAAAGC1409.311156E-619.7117253
TAATAGT950.00782544118.1555354
GATCGTT950.00782544118.15553511
TATTAGA950.00782544118.1555352
GTGTAGC950.00782544118.15553511
AGGATAG1250.001934828816.56087969
ATTAAAC1250.00193719816.557853
GTATTGA1300.002517300415.9210091
Produced by FastQC (version 0.11.2)