FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845048
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23640.27974742263161384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1101.1525717E-625.0899188
AGACCGC1101.1531665E-625.0884346
TAGGTAG1906.7484507E-1021.7873235
GACCGCG850.004121646320.2921167
GGCTCGG1258.748128E-519.316953
ACGCCGA900.00572273819.17271857
CCGGCCC950.00783456318.1571778
CTAATAC3103.6379788E-1217.8036423
CCCGGCC1402.0823823E-417.24932110
GTATATA4800.017.2482991
CCGGGGC1653.844848E-516.7246324
CCTAGCG1452.724623E-416.65254430
GCTCGGA1503.5279852E-416.097464
ATTATTC5000.015.8674963
TATACTT4150.015.7936245
TAGACCG2452.9182047E-715.4882685
CGGGAGC2051.439982E-515.14843318
GGCGAGT1605.7497935E-415.09583512
CGGCCCG1605.757549E-415.0931549
TCTAATA4001.2732926E-1114.6601872
Produced by FastQC (version 0.11.2)