FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161921
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110540.21414508280928746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA24000.016.0994951
GTATTAG20100.015.9621851
CGCACCT10400.014.92857266
GGCTCGG6500.014.8615045
CTAATAC22900.014.6127943
TAATACT31650.014.6060344
TATACTG31550.014.1061485
GTATAGG17800.013.95461751
TCCGCAC10600.013.67070664
CCGCACC11150.013.615108565
TATACAG26650.013.5928385
TAGGTAG11600.013.383595
GTCCTAG8050.013.2852961
GTACTAG15750.012.9234031
CCTAATA22200.012.8978692
GTATATA36550.012.8368061
TCTATAC22150.012.7713593
ACTATGC23150.012.5184498
CTCGGAG9800.012.321287
TATTAGG18200.012.32068352
Produced by FastQC (version 0.11.2)