FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442590
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59600.1731254665818468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14050.017.9239541
TATTAGG13600.017.2484862
TAGGTAG8100.016.6106175
CTAATAC15700.015.8207283
TATACAG18000.015.7161995
GTATATA25900.015.0510211
GTATAGA13600.014.9658161
GCTCGGA6900.014.4997816
TATACTG21800.014.4009495
GGCTCGG6250.014.3515745
TAGTCAG7500.014.2599919
TTAGGTA11500.014.0991734
CTATGCA14450.014.0864969
TCCGCAC6250.013.80099364
GTCTAGG8450.013.4723851
CTTAGAC9850.013.3088623
ACTATGC16000.013.15292558
TAATACT22900.013.1062174
TATATAC27350.012.9919243
CCTAGCG5053.6379788E-1212.97849330
Produced by FastQC (version 0.11.2)