FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900138
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56930.19630100360741454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA12100.019.3882221
TCCGCAC5800.018.44104864
GTATTAG11050.018.1084021
GTCCTAA6850.017.6275481
GTACTAG10300.017.082341
CCGCACC6600.016.72824765
CTAATAC11700.016.5115053
GTATAGG10650.016.197011
CTATGCA12650.016.0910139
TAGGTAG6500.015.9226315
CCTAATA11900.015.6539432
CGCACCT6900.015.50063566
TATTAGG10700.015.4751362
CCTACAC5900.015.2021783
TATACAG14600.014.8865695
CTGTGCG3554.6929927E-1014.5775599
TAGACTG6450.014.4414565
TTATACT14000.014.2919644
CCTCGCG2708.7722947E-714.0556479
CGATCAC5400.014.0539524
Produced by FastQC (version 0.11.2)