FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5503713
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105670.19199765685456344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25050.022.7249811
TAGGTAG14650.020.958875
CGCACCT12000.019.83787766
TCCGCAC12200.019.79617764
TAGTCAG13450.018.211919
CCGCACC13000.018.04682465
TATTAGG22850.017.8158842
CTAATAC26300.017.7088173
TTAGGTA22250.015.8154554
TAATACT36650.015.531794
GGTAGTC16900.015.3103857
TATACTG37450.015.2001415
GTCCTAG8600.014.8433051
CCTAATA24700.014.805412
ACTATGC26900.014.7490838
GTATAGA26800.014.5469181
TATACAG29750.014.4956795
CTATGCA22800.014.3750119
ACATAGG15900.014.10250331
GTATATA47250.014.0923311
Produced by FastQC (version 0.11.2)