FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5752232
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99570.1730980252535016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22750.019.25771
CTAATAC25450.017.621443
TCCGCAC13350.017.57428664
GTATAGA26700.016.9255241
TAGGTAG13700.016.8714125
CCGCACC14200.016.03607665
ACTATGC24150.015.9994728
CGCACCT14050.015.96171566
TATACAG32450.015.8404965
TAATACT36100.015.7674924
TCTATAC26950.014.208563
TATTAGG22700.014.1331582
CCTAATA27050.013.9008492
CTAGGAC9200.013.8739393
CTATGCA21900.013.3899699
GTATATA44550.013.2412961
TACACCG12250.013.2360685
TATACTG33650.013.1226665
GTATAGG19850.013.0341551
TAGACAG20650.012.6966975
Produced by FastQC (version 0.11.2)