FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284229
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42000.18386948068691888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5400.018.5268555
CTAATAC10450.018.4867133
CGCACCT4550.018.19888966
GTATTAG9700.018.1374781
TAATACT14850.017.4226114
TCCGCAC4650.017.06628264
CCTAATA9750.015.9212382
ATTAGGT8050.015.8560063
GTATATA16800.014.9896691
TAGTCAG4900.014.78562459
GCGCACT3051.4662874E-814.70515111
TATACCG6350.014.6685615
CCGCACC5500.014.42813365
TATTAGG8900.014.3410412
CCCCGGG1450.005211876714.27796512
TACACAG8500.014.2051755
TATACTG14200.014.0908475
ACTGTGC6000.013.7999178
TATATAC19650.013.6936743
TACACCG5050.013.6626865
Produced by FastQC (version 0.11.2)