FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330575
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82040.24632383297178415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13150.024.9237861
TAGGTAG8500.023.9468965
GTATAGA16800.018.482021
TAGTCAG9000.017.6337439
CCTAGCG5450.016.45736930
TCCGCAC6300.016.42944764
CTAATAC15750.016.4282153
GGTAGTC12300.015.9881767
TATACAG18400.015.7498945
TATTAGG16450.015.7289042
CCTAATA15800.015.720942
ACATAGG10250.015.48142831
TTAGGTA13400.015.4474254
CTAGGCA6300.015.33299910
GACGCGC4800.015.09228838
CGCACCT6700.014.93363166
TATACGG12500.014.9038995
CTAGGAC5250.014.4568283
ATTAGGT15650.014.3287613
TCTATAC14500.014.2755513
Produced by FastQC (version 0.11.2)