FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015925
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103290.20592413164072432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC9700.019.91823664
GTATTAG23350.019.6516651
TAGGTAG15000.018.6297345
GGTAGTC16250.017.6214667
CGCACCT11050.017.17238666
TAGTCAG14150.017.0668959
CCGCACC11450.016.87379665
GGCTCGG7000.016.7569075
ACATAGG15850.016.10606631
TATTAGG25500.015.15257552
CATATAC36250.014.6564893
TACACCG10350.014.3331315
TATACGG20950.014.3267665
CTAATAC25900.014.1196333
TTAGGTA21200.013.9952244
TATACAG35850.013.8575445
ACTATGC25450.013.6913598
GTATAGA26900.013.4670231
TATACTG34950.013.3259925
AGTCAGA18900.013.32494210
Produced by FastQC (version 0.11.2)