FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400574
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88620.201382819604897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC8150.019.89721764
GTATTAG20150.017.975971
CCGCACC8950.017.7331965
CGCACCT9600.016.89190766
TAGGTAG10750.016.3664535
GTATAGA23300.014.9535221
GTATAGG15200.014.297991
CTAATAC22500.013.9523313
GTATATA53200.013.746821
TAATACT36250.013.3231824
TAGTCAG11000.013.1722319
ACTATGC21200.013.018088
TATACTG31750.012.9299275
TATTAGG22500.012.8787822
TATACAG30100.012.7218225
TATACCG14550.012.3291525
TATACGG17100.012.3062745
TTCCGCA17550.011.79576863
GCTCGGA5853.6379788E-1211.7941596
CTAGACA11750.011.7438364
Produced by FastQC (version 0.11.2)