FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847872
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74610.19389938126839978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8100.016.1845845
GTATTAG17450.015.618941
GTATAGA20000.015.0075281
GTCCTAA8950.014.6480721
GGCTCGG5250.014.4566065
TATACCG10750.014.4413245
TATACGG14900.014.1236375
CTCGGAG6400.014.0151057
CGCACCT7000.013.800384566
CCGCACC6300.013.69103665
TCCGCAC6500.013.26977364
CTATACT18100.013.1512754
GTATAGG14800.013.0540791
GTATTAT34750.013.0057791
GCTCGGA6700.012.8726566
TATTAGG18100.012.7700792
CATATAC24000.012.6493653
TATACTG24900.012.6079665
GTATATA42100.012.5380291
TCTATAC18950.012.3793263
Produced by FastQC (version 0.11.2)