FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785422
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114320.19760010592140037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27150.020.9662511
TAGGTAG16850.020.269455
CGCACCT11450.017.47699466
TCCGCAC11600.017.25164
CCGCACC12100.016.25300665
GGCTCGG8100.015.7588085
CTAATAC28650.015.5331843
TAGTCAG16800.014.9907729
TAGACTG13350.014.7299175
GTATAGA28450.014.6726911
GGTAGTC17500.014.5881567
TATTAGG32300.014.311792
GCTCGGA9600.014.0152246
GTATATA69700.013.463031
TATACAG40450.013.39022355
TCTATAC26450.013.0427683
TATACTG38400.013.0269725
CTAGGAC8250.012.962933
GGGCTCG9400.012.8450444
ACATAGG18650.012.76271731
Produced by FastQC (version 0.11.2)