FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857530
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82370.2135304197245388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC18150.018.0572223
GTATTAG16750.017.9183161
TCCGCAC7650.017.13843764
CGCACCT8500.016.64189166
TAGGTAG9400.016.5158125
CCGCACC8450.016.33227365
GTATAGA17800.015.6984851
TAATACT28700.014.4245854
ACTATGC17450.013.8395998
CTATGCA14900.013.6611319
TCTATAC16750.013.5935573
CCTAATA19600.013.5525722
TATACTG23950.013.5405895
GTATATA41400.012.8325541
GGCTCGG5400.012.7776655
TATACGG15950.012.7616435
TATTAGG19000.012.7095912
TATACCG11700.012.6793765
GACGCGC3852.2870154E-812.54420638
ATTAGGT18800.012.29478553
Produced by FastQC (version 0.11.2)