FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5335211
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90860.170302542861004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT10250.020.53306866
GTATTAG24550.019.5323371
TAGGTAG12650.019.0902065
TCCGCAC11200.018.79195464
CCGCACC12550.017.59479165
CTAATAC28100.015.5912233
TATTAGG24700.015.0834762
GGCTCGG6650.014.5251013
GTATAGA26800.014.0308051
TATACAG33450.013.7169855
TTAGGTA23150.013.5605484
TAATACT40200.013.4728794
TATACTG35200.013.3290555
GGTAGTC16150.013.2440947
CCTAATA28850.013.1528992
GTATATG33900.013.0257021
ACTATGC25000.012.9717658
TAGTCAG14900.012.9664529
GACGCGC6200.012.79695638
CCTAGCG7850.012.74367830
Produced by FastQC (version 0.11.2)