FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186472
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127790.3052450846440631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG5700.017.551543
GTATTAG19300.016.2662161
TATACAG25650.014.9292555
GTATAGG15050.014.4413071
GCTCGGA7300.014.17724
GTATATA38500.013.7994721
CATATAC27750.013.55050853
CTCGGAG7900.013.1007645
TCTATAC20050.012.9044353
GTATAGA20500.012.62146951
GTATATG23000.012.5995181
CTAATAC19400.012.2698563
TATACTG29900.012.1149225
TATACGG16150.011.9623915
CCTAGCG5853.6379788E-1211.79357530
TATTATA40750.011.7673662
TAGGTAG10850.011.7645265
GGGCTCG8300.011.6378322
CCTAATA18350.011.4679522
TATATAC44550.011.4605673
Produced by FastQC (version 0.11.2)