FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454841
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106452.3403782860384177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT1700.028.4117269
GGTGCGT500.007725994627.6029938
ACGCGTG2750.027.59995769
ATTCCGT851.4403679E-424.3555816
TACGCGT4450.024.03366969
TTAGGTA1151.6939102E-623.9999644
CATTCCG750.001991199322.9999645
GCCTCAC902.1236317E-422.99996420
CCTGCCC902.1236317E-422.99996410
TAGACAG1554.072899E-822.258035
ACTTAGT953.0571627E-421.7942311
TAGGTAG953.0617134E-421.7894385
AAGACCG1154.5948367E-520.9999685
GTATTAG1851.1687007E-820.5179941
CCGGGGC1258.718961E-519.32209212
CCCTAAA1258.72633E-519.319972
TTACGCG5750.019.19996868
ACTAGCG900.005729253419.16663644
TATAGAG1651.8234332E-618.8181515
CGAGGTT950.00783736718.15387329
Produced by FastQC (version 0.11.2)