FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4974265
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131480.26432045739420795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13100.018.1717855
GTATTAG22800.017.7032621
TCCGCAC8700.017.05260864
CCGCACC9150.015.83672865
GGCTCGG6100.015.8360925
GGCGTCG6850.014.10093732
TAGTCAG12350.013.9676339
GGTAGTC14600.013.9418037
GCTCGGA7250.013.8000226
CGCACCT10800.013.73640866
GTATATA64750.013.3732291
TATTAGG27950.013.2067382
CTAATAC25400.013.1746883
TTAGGTA21750.013.165144
TCTATAC24100.012.883323
ACATAGG14150.012.67744631
TATACCG17300.012.36418155
GTATAGA25600.012.2631981
GTATAAT37200.012.0559861
GTAGGAC8050.011.99953653
Produced by FastQC (version 0.11.2)