FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082580
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88650.21714210131828404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19650.018.4345051
TAGGTAG10850.017.8058645
GTATAGA20350.016.2746471
GTATAGG14550.015.6489691
TATACGG15500.015.3575565
CGACGGG3554.6929927E-1014.57769849
TAGTCAG10300.014.0676699
GGCTCGG4202.7284841E-1113.9638245
ACCGCGC2751.0794829E-613.7997138
TACGGTG13300.013.7478347
TCCGCAC6600.013.59162664
ACGGTGT13600.013.4445738
ATACGGT15950.013.1939226
GGTAGTC11350.013.0702147
TATACTG27800.012.9060495
CTAATAC20100.012.8720793
CCTAGCG4852.3646862E-1112.8027630
GTATATA53650.012.7964091
TCTATAC20300.012.5753233
ACATAGG11450.012.35239631
Produced by FastQC (version 0.11.2)