FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010656
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108490.21651855565418981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC8800.021.56367365
TCCGCAC8650.020.74101464
TAGGTAG13700.018.3829275
CGCACCT10800.017.25093866
GTATTAG24600.016.5490191
GGTAGTC15800.015.9397867
CTAATAC25600.015.6321033
TAGTCAG13950.015.3334039
ACATAGG14400.015.09246331
TTAGGTA23300.014.0658854
GTATAGA25600.013.4767581
GTATATG34650.013.3421841
TATACAG37550.013.2301555
TATTAGG28500.013.0729592
TATACCG15200.012.710335
TATACGG19300.012.5127595
TATACTG37300.012.4864025
TACACAG16250.012.3136555
GTATAGG16850.012.2850441
CCTAATA30600.012.0630572
Produced by FastQC (version 0.11.2)