FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186042
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89220.2131368963808772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10950.016.6985055
GTATTAG17750.016.1316951
TCCGCAC6800.015.73007964
GGCTCGG4900.015.489685
ACCGCGC2705.259062E-815.3332198
CGCACCT7650.014.88437766
CCGCACC7250.014.7537365
GGTAGTC12950.013.8531797
GCTCGGA5750.013.7998986
CTAATAC20450.013.4959113
GACCGCG2609.172214E-613.2691327
GTATAGA21500.013.1575711
TATACAG29200.012.9964795
GTATATA55600.012.9679631
CCTACAC7750.012.9092733
TATACTG29900.012.8075975
TAGTCAG11200.012.629229
GTATATG29100.012.5664671
GTCCTAG5501.8189894E-1212.5449111
TATACGG18000.012.2665765
Produced by FastQC (version 0.11.2)