FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677383
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77830.2116450747719234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10100.017.7619575
TCCGCAC7500.017.48077464
GGCTCGG3850.017.025575
CTAATAC17800.016.6676923
CGCACCT7250.016.65591266
CCGCACC7300.016.54182865
GTATTAG15650.016.3135991
GACGCGC3351.9645086E-1015.44655638
GCTCGGA5150.014.067826
TATACGG15700.014.0633615
TACGGTG12250.013.7998627
CTATACT17250.013.7992984
ACGGTGT11550.013.7401218
CTCGGAG5700.013.3156567
TACACAG11550.013.1425475
GGTAGTC11050.013.112997
CTAGGAC4508.913048E-1113.0326713
ATACGGT15750.012.9236786
TTAGGTA17250.012.799354
TATACTG26000.012.738165
Produced by FastQC (version 0.11.2)