FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316229
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87350.2023757312227873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC8050.019.71623665
TCCGCAC8100.019.16900664
CGCACCT8300.019.12215266
TAGTCAG11200.018.1735889
GTATTAG20900.018.1575871
TAGGTAG13150.017.8400025
TATTAGG22050.015.645452
GGTAGTC13550.015.2763167
TATACTG28250.014.7767395
CTAGGAC6250.014.3512573
TATACAG29100.014.3451175
GTATAGA22750.014.1030571
GTATATA51750.013.99976351
ACATAGG13650.013.89957531
CTAATAC23400.013.8582573
GTATAGG15200.013.845161
TAATACT33250.013.0730084
GACGCGC4255.002221E-1012.98681138
ATTAGGT22850.012.9840113
TTAGGTA20150.012.8405274
Produced by FastQC (version 0.11.2)