FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193674
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106130.2530716502999518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC8650.018.3490164
GTATTAG18600.017.9908621
TAGGTAG11850.017.7588585
CCGCACC9650.016.80471265
CGCACCT9950.015.60431866
CTAATAC22550.014.3803463
CCTAATA22350.014.0459752
GCTCGGA5200.013.9316954
TATACGG17350.013.7199715
GGCTCGG5050.013.662393
CTAGGAC5850.013.5631323
GTATATA54150.013.1238711
TACACCG8700.013.0857385
TCTATAC20700.012.8324153
GTATAGA19450.012.7704471
GTCCTAA10000.012.7642391
TAGTCAG10900.012.6602489
TAGGACT7650.012.6265474
TATACAG29000.012.4909335
GGTAGTC14850.012.3126917
Produced by FastQC (version 0.11.2)