FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579581
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146680.5686194773492285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT4150.022.44488169
ACGCGTG5750.019.79920469
CCGCGCG2608.913048E-1118.5768979
TCCGCAC4750.017.43155564
TAGGTAG6500.016.4538235
CCGCACC5050.016.39601765
TACGCGT9550.016.25589269
TAGGACT5200.015.9221294
CGCACCT5400.015.33331266
CTAATAC13850.015.1940413
GTATTAG11950.015.0125321
TTACGCG11950.015.0119569
TCTATAC12400.014.1886713
ACTCCGC4403.6379788E-1214.1136168
GCTCGCG3202.7563146E-814.01669361
GCTCGGA3708.731149E-1013.9864666
GCCCCCG2506.1090104E-613.80131820
CTAGGAC4505.456968E-1213.7991783
TATACGG9900.013.5908915
CCGTGCG1550.00811416713.354829
Produced by FastQC (version 0.11.2)