FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060203
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182410.5960715678012211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6300.019.7134385
TCCGCAC5000.017.2512364
TAGTCAG7100.017.0071439
CCGCACC5300.015.623754565
CTAGCCT5650.015.2645794
GTATTAG13200.015.1584371
TATACAG19450.014.544365
GCTTAGG11650.014.50986869
TATTAGG15150.014.3459162
GGCTCGG4202.7284841E-1113.9636835
TATACCG8100.013.6290425
GTATAGA14300.013.2686591
GGTAGTC8250.012.9635017
GCTCGGA4551.07320375E-1012.8899766
CTAATAC15050.012.8366563
CTAGGGG2255.3670054E-412.2661383
ACAGCGT7650.012.1765438
TATACGG12850.012.0811915
GGCTTAG17500.012.02569
CATATAC21900.011.9720873
Produced by FastQC (version 0.11.2)