FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785688
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162590.33974216455397843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC8250.015.89151564
GTATTAG23700.014.9933031
GGCTCGG7550.014.6224625
CTAATAC23450.014.2704383
CCGCACC9300.014.09731365
TAGGTAG12250.013.799955
GCTCGGA8300.012.4698346
TATACCG15800.012.4461575
GTATATA59900.012.4404291
TAATACT41050.012.186064
TATACTG35050.012.1069465
TATTAGG23500.012.0380022
CCGGCCC4308.218194E-912.03597313
GTATAAT34450.012.0171221
GTATAGA24350.011.7594641
CGCACCT11350.011.55098166
TAGTCAG11150.011.4483889
GTCTTAG12700.011.4091691
TATACGG17700.011.3050435
CCTAATA25450.011.1156412
Produced by FastQC (version 0.11.2)