FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704903
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185420.5004719421804025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8500.019.8881235
GTATTAG15350.018.2048631
TAGTCAG8400.014.37511359
CTAATAC15850.014.3652673
TATTAGG16400.014.3042172
TCCGCAC7400.013.98735164
TAATACT26750.013.5414314
GTATAGG12300.013.1826741
GCTCGGA6150.012.9025396
ACTATGC16400.012.8324178
GGTAGTC11300.012.823117
TATACGG12950.012.7875725
CATATAC24700.012.7099393
CCGCACC8250.012.54622965
TATACCG11300.012.5176295
CGCACCT8000.012.50702366
CCTAATA18850.012.4450492
TACGGTG11650.012.4378657
TTAGGTA14200.012.3902744
ACGGTGT11600.012.1940618
Produced by FastQC (version 0.11.2)