FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3747861
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229870.6133365138141462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG6500.016.4537265
TAGGTAG8100.015.7591455
GCTCGGA7150.015.4404466
GTATTAG16850.014.7417321
CTCGGAG7850.014.5030787
TATACGG13000.014.3306645
TATACAG22750.014.1031925
CTAATAC17750.013.6051713
GTATATA39750.013.365941
GTATAGA17600.012.9374061
TATTAGG17350.012.9246322
CGTGGGC5900.012.8643121
CCGCACC6250.012.69658565
GTCTTAG9250.012.6809881
TACACCG7400.012.5877465
TCTAGAC9650.012.5125273
GGGCTCG8300.012.4694554
CCTACAC7050.012.2336253
CTTACGT14950.012.230679569
CGGGTCG2852.3565728E-512.10533618
Produced by FastQC (version 0.11.2)