FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017462
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131180.4347362120881721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG1602.9541916E-517.2491347
ACCGCGC1855.4545744E-616.7832188
AGCGGGG3703.6379788E-1215.85160750
GCGCACT4051.8189894E-1215.33408711
TATACAG21150.014.3538895
GTATTAG12950.013.8532751
GTATAGA13700.013.8503581
TCCGCAC5500.013.8006864
TCTAGAC6750.013.7993083
GGACCGC2001.9839821E-413.7993086
TATACGG10850.013.6721255
TAGGTAG7150.013.5098125
CCGCACC5150.013.39871965
GGTGCGA6700.013.38694528
CGACGGG3651.0824806E-813.2330949
GTATATA36850.013.1071861
CCGGCCC3952.3228495E-913.1019113
TATATAG25500.012.5817225
ATACGGT10150.012.5757246
TATACCG11000.012.5448265
Produced by FastQC (version 0.11.2)