FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605986
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62630.3899784929632014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7400.018.6487481
TCCGCAC3058.58563E-1015.8376364
CCGCACC3058.58563E-1015.83713765
CGCACCT3151.3569661E-915.33437166
TAGGTAG5250.015.1148385
TAGTCAG4600.015.0005489
TATTATA16900.014.9015212
GGCTCGG2353.2203207E-614.6804743
GGTAGTC5300.014.3212797
ATAGGAG5100.014.20463333
GTATTAT13750.014.0509841
ACATAGG4700.013.94558131
CGTGGGC2506.1112587E-613.8000751
GTATAAT10000.013.8000751
GTATAAC5050.013.663441
TCTACAC5050.013.6630143
GTCAGTC1550.00811038413.35532713
CTAATAC7500.013.3396573
TAGGGGT2355.23004E-513.2124274
TATTAGG8100.013.2029532
Produced by FastQC (version 0.11.2)