FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186235
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105570.8899585663886161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG2105.0353265E-818.0720375
TAGGTAG3250.018.046765
CCGACGT1200.001476899817.24985340
CTCGGAG2251.10720066E-716.8672337
GTATTAG4950.016.725721
GCTCGGA2301.422286E-716.5005556
GTATAGG3351.0913936E-1116.4760821
TAGTCAG2351.8158244E-716.150169
GGTAGTC3502.1827873E-1115.7719587
TATAGGG2705.2508767E-815.3325562
CGTGGGC2051.4441108E-515.1449341
GGGCTCG2554.5944398E-714.8815994
TATACTG6500.014.8620395
TATACGG4201.8189894E-1214.7862125
CTTACAC2353.219442E-614.6801053
ATCTTAG3101.816261E-814.4663991
GTGTAAG3951.5643309E-1013.9733861
GTATTAT8400.013.9629891
TATTAGG5700.013.9203472
CGGGAGT1500.006527543513.80046348
Produced by FastQC (version 0.11.2)