FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741514
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115030.6605172281130097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG4100.026.92567469
CGCGCGA700.001334678724.64109240
GGCTCGG2800.019.713443
TACGCGT8000.018.11172569
GCTCGGA3051.8189894E-1218.0975844
TTACGCG10000.017.93923269
ACGGTGT5100.017.5879868
CCCCTAG1200.001478183817.248271
GTATAGA7100.016.519471
CTCGGAG3753.6379788E-1215.6397795
GGGCTCG3351.9645086E-1015.4462122
TACGGTG5400.015.33311757
TCCGCAC3201.6934791E-915.09570464
CGCGTGT2756.582195E-815.05390169
GTATTAG7750.014.6888491
CCTATAC5900.014.6180183
TATACGG7200.014.3747975
TAGAGTG4650.014.0965765
CATATAC13950.013.8488683
TGCGCGC1500.00652998313.80020120
Produced by FastQC (version 0.11.2)