FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454078
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4450.018.6077445
TATACTG9500.018.1588735
GCGTCAG3700.017.7177814
TATTATA8450.017.5577622
TCTATAC7050.017.1291713
CTAATAC6100.016.968713
GTAGGCT2251.10701876E-716.8681553
GTATAGA5700.015.7387711
GTATTAT10200.015.5607311
CGACTAG1808.08876E-515.33310415
GCAGCGT4750.015.2545011
GTATTAG5600.014.7875271
CCTAGCG2101.8117289E-514.78651130
TAGGTAG2808.1958206E-814.786515
TTCTAGG4001.2732926E-1114.6637932
TAATACT8300.014.5494754
TCTAAGC4353.6379788E-1214.2771213
TCGGCAC2901.260214E-714.27564952
CCTAATA6050.014.2574562
TAATACA7300.014.1793344
Produced by FastQC (version 0.11.2)