FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955657
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10800.017.2525674
GTATAGA20100.016.3092211
GTATTAG14600.016.3080441
TATACAG18900.016.2482435
CTAATAC16950.015.0641883
TAATACT26350.014.6663184
GTATATA29150.013.9684781
GCAGCGT14000.013.8027511
TATTATA25250.013.8020532
TAGACTG9550.013.7296185
CCTAATA15750.013.5829732
TATACTG24150.013.5732765
CAGCGTC14000.013.5555882
ACGCGCA5700.013.31558239
TAGGTAG8150.013.1244855
ATTATAC20950.013.0114823
GATCTCG13100.012.9043838
GTATTAC16450.012.7958631
CGCGCAT5700.012.71032840
TATTAGG15250.012.4444742
Produced by FastQC (version 0.11.2)