FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5408541
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14050.022.3478744
GTATTAG23950.018.4418161
CAGCGTC18100.017.9196052
GCAGCGT18400.017.6282521
CTAATAC24800.017.3915183
CGCACCT12000.015.23721166
TCCGCAC12350.015.08473664
CCTAATA23800.014.6427812
AGCGTCA22150.014.487333
TATACTG33300.014.2990095
TATTAGG22700.014.1363162
TAATACT40450.014.07461554
TATTATA43050.013.8660292
CCGCACC13250.013.7997465
ATGTGTA25300.013.77246811
TAGGTAG13500.013.5460675
GATGTGT24250.013.37306610
GTATAGA24700.013.2716791
GTATTAT39600.013.2448671
ATTATAC30000.012.8817813
Produced by FastQC (version 0.11.2)