FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192141
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9400.019.4537644
GTATAGA20500.017.8418031
GTATTAG17750.017.3012851
CTAATAC17050.016.3919943
TAATACT30750.015.5964354
GCAGCGT11850.015.4327831
CAGCGTC12750.014.6135182
TAGGTAG10050.014.0758115
CCTAGCG6250.013.8000130
GTATATA34300.013.782031
TATTATA36550.013.593972
TCCGCAC8150.013.54586164
TATTAGG18300.013.1983052
GTATAGG12900.013.1066921
GTCCTAA10300.013.0651431
GTGTAGG8750.013.0134551
GTATTAT30750.012.90444851
ACCGTGC5700.012.7103838
TATATAC37800.012.596753
ATTATAC26650.012.5587293
Produced by FastQC (version 0.11.2)