FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225644
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG3800.025.4246754
GCAGCGT4700.020.556961
CAGCGTC4900.019.7170962
TGACGCG1852.6439557E-718.64826437
CGCGCAT1753.2087792E-617.7424940
AGCGTCA5650.017.0997813
CTAATAC4500.016.8690723
ACGCGCA2057.7566983E-716.8289239
CCTGCCG1653.8426082E-516.72692716
GTGTGAG2301.4232864E-716.4996599
TAGGACA2753.7180143E-916.3114154
GATGTGT6150.016.26795610
TGTATAC6000.016.1022953
GTGTATA6000.016.09966713
GACGCGC2151.2756136E-616.04617938
TAGACAG3703.6379788E-1215.8536115
GTAGGAC1756.3494175E-515.7736773
TATACGG3502.1827873E-1115.7736775
CGTCAGA6200.015.5828655
ATGTGTA6900.015.49967911
Produced by FastQC (version 0.11.2)