FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872095
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG5500.018.8186194
TAGGTAG4650.018.5488195
TAGGACT3600.017.25044
GATCTCG6400.016.71087838
GTATTAG7450.016.673311
TATACTT10800.016.6114985
CCGTACG2109.983305E-716.4285149
TGCGCGG2109.983305E-716.42851434
CTAATAC7000.016.2646643
GCAGCGT6600.015.6838591
TATACAG8000.015.525365
GTATTAT11850.015.4323861
CAGCGTC6950.015.3888462
GTCCTAA4300.015.246171
ATATACT12700.015.2129524
GTATAGG7850.014.9446191
GTATAGA7900.014.8500331
GTATTAA6900.014.5018861
TAGGCTA2152.2638631E-514.4421975
TACACCG4101.8189894E-1114.305215
Produced by FastQC (version 0.11.2)