FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72046
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1650.22902034811092914TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1270.17627626793992726RNA PCR Primer, Index 1 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG810.11242817089081976TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG790.10965216667129334TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAC150.002346567669.0000167
TAACATG150.002346567669.000015
GGGTTAA150.002346567669.000011
AGGGGGG309.039824E-657.50000469
ACCGTCC252.5510075E-455.256
CTTCGCC200.007331251.75000453
CAGCGCA200.007331251.7500049
TGTGTCT200.007331251.75000436
TACTCCG350.001339427839.4285745
GTATAGA400.002580271334.51
AAGGGGG905.0476956E-934.569
ACTCCGT400.002580271334.56
AAAGGGG905.0476956E-934.568
CATACCA450.004594267330.6666664
TGCAGTT450.004594267330.66666665
TCAAAGA450.004594267330.6666663
TAATATC703.8016726E-529.5714284
CCGTCTT1601.8189894E-1228.03125247
GCCGTCT1601.8189894E-1228.03125246
TACGATA500.007687650627.619
Produced by FastQC (version 0.11.2)