FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653321
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA14950.017.7725621
TATCTCG16750.017.3014236
GTATTAG13050.016.9227121
TAATACT20650.016.2072964
TATTAGG11900.015.9470592
CTAATAC13700.015.8666343
TGCCGTC17600.015.28970845
CCTAGCG5650.015.26563330
GCGTCAG9500.015.2540284
CTATCTC19850.014.94704135
GTATGCC18750.014.71993842
CCTACAC6150.014.5869023
CTACACA9250.014.5472794
CCTATCT20450.014.50849734
CGCGCAT4353.6379788E-1214.27580240
CTCGTAT19200.014.19525439
TCTCGTA19850.013.90422338
ATCTCGT20600.013.90042837
TATACTG21100.013.8993785
TACACAG11950.013.8590115
Produced by FastQC (version 0.11.2)