FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826952
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.020.69983745
GTATAAA2201.891749E-1020.3899021
TGCCGTC4100.019.35350647
ATGCCGT4300.018.45334246
GCCGTCT4000.018.11235848
TATAGGA1954.5837805E-717.6943072
GCCTAAT1402.0789294E-417.2529931
TATAAAA1804.1887943E-617.2519512
TATAGTG2401.22763595E-817.2509085
TATATCG2251.10578185E-716.8675545
ATAGGAC1653.8357713E-516.7291643
TATAGCA1704.9533846E-516.237132
CTCCTAG2351.811859E-716.151741
GTATAAT1503.5189805E-416.1027931
ATAAGGG2603.2912794E-815.9248773
GTATTAG2201.616243E-615.684541
CTATATC2907.508788E-915.4663314
CGTATGC4700.015.41477243
GCTATAG1350.003238270315.3359941
TATTATA1350.003239574815.3350672
Produced by FastQC (version 0.11.2)