FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895344
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG3200.018.330344
ACGCGGT2359.493306E-917.61682751
GTATAGA7650.017.5917531
GTATTAG6100.016.9706061
CCGCGCT2251.1088014E-716.8664835
TATTATA7150.016.4080092
GTATATA9100.016.3054581
TATAATA6150.016.2706892
ACCCGCG2201.6220511E-615.68164433
GACGCGC1851.0219659E-414.91875438
TATGCGG3051.4662874E-814.70475557
GTATGCG3605.7843863E-1014.37484156
ACGCGCA1951.599315E-414.15368939
CGCGGTA3454.9039954E-913.99984552
GTATAAA7650.013.9831891
TATACAG8650.013.9612255
GTCTTAA5950.013.9187491
CGCGTCA1750.00113541513.79984837
GTATTTA8600.013.6422631
TAATAGT7600.013.6200674
Produced by FastQC (version 0.11.2)